Tujuan penelitian ini adalah mengeksplorasi Pedagogical Content Knowledge (PCK) mahasiswa jurusan Biologi pada mata kuliah microteaching yang dibantu supervisi klinis. Penelitian ini menggunakan metode kualitatif dengan subyek penelitian 26 mahasiswa yang berasal dari rombel 1 dan rombel 3 prodi Pendidikan Biologi peserta mata kuliah microteaching pada semester genap 2015/2016. Data dikumpulkan dengan instrumen CoRe dan PaP-eR serta observasi. Supervisi klinis dilakukan dari awal perencanaan pembelajaran yang diajukan hingga pelaksanaan praktek mengajar masin-masing mahasiswa. Hasil rekapitulasi rubrik penskoran CoRe melaporkan bahwa mahasiswa digolongkan ke dalam kategori tengah dan bawah. Analisis PaP-eR menunjukkan bahwa mahasiswa masih lemah pada penguasaan materi, manajemen waktu dan penggunaan media yang tepat dalam pembelajaran.

The purpose of this research was to explore Pedagogical Content Knowledge (PCK) of Biology students in microteaching class assisted by clinical supervision. This study was conducted by qualitative method with 26 students of microteaching class from 1st and 3rd study group majoring in Biology teaching in even semester of academic year 2015/2016 as the research subject. Data was collected by CoRe, PaP-eR and observation. Clinical supervision had been conducted since the beginning of the class when students planning the lesson till their each teaching practice. Recapitulation of CoRe scoring rubric reported that students were categorized in midlle and bottom group of PCK. PaP-eR analysis showed that students were still having low competences in content mastery, time management and using appropriate learning media.